The release of alanine by rat diaphragm muscle in vitro.
نویسندگان
چکیده
Alanine release by rat diaphragm muscle in vitro is stimulated by glutamate, valine, leucine and glucose. The stimulation by glutamate and valine (but not leucine) is inhibited by 3-mercaptopicolinate. These results suggest a metabolic route involving phosphoenolpyruvate carboxykinase which directs amino acid carbon skeletons towards pyruvate synthesis for alanine formation.
منابع مشابه
Origin and possible significance of alanine production by skeletal muscle.
These experiments were undertaken to determine the source of alanine released by skeletal muscle and to clarify the possible relationships between this process and the degradation of branched chain amino acids, the release of glutamine, and protein turnover in this tissue. During incubation in vitro, the rat diaphragm underwent net protein breakdown and released amino acids into the medium at a...
متن کاملLinkage of aerobic glycolysis to sodium-potassium transport in rat skeletal muscle. Implications for increased muscle lactate production in sepsis.
Although a linkage between aerobic glycolysis and sodium-potassium transport has been demonstrated in diaphragm, vascular smooth muscle, and other cells, it is not known whether this linkage occurs in skeletal muscle generally. Metabolism of intact hind-leg muscles from young rats was studied in vitro under aerobic incubation conditions. When sodium influx into rat extensor digitorum longus (ED...
متن کاملENDOGENOUS RELEASE OF OPIATES BY REPETITIVE ELECTRICAL FIELD STIMULATION IN THE GUINEA-PIG AND RAT ILEAL LONGITUDINAL MUSCLE
The effect of repetitive electrical field stimulation and the response of the guinea-pig and rat ileal longitudinal muscle to single pulse stimulations was examined. Single pulse field stimulation produced twitch contraction which was inhibited by repetitive field stimulation (10 Hz, 40V, 0.5 msec for 5 m). This inhibition was largely, though never completely, reversed by naloxone. Contrac...
متن کاملEffect of New Derivatives of Dihydropyridine on Rat Ileal Smooth Muscle in Vitro
In this research we evaluated the calcium channel antagonist activity of various diester analohues of nifedipine on rat ileal smooth muscle.in these analogues,the orthophenyl group at position 4 was replaced by 1 methyl 2-meythylsulfonyl or methylthio 5-imidazolyl.wistar rats(180-250g) were killed by a blow to the head.the intestine was removed above the ileucecal junction and longitudinal smoo...
متن کاملEffects of Black Scorpion Androctonus crasicuda Venom on Striated Muscle Preparation in vitro.
Effects of venom from black scorpion Androctonus crasicuda (AC) were determined on isolated chick biventer cervices nerve-muscle and mouse hemidiaphragm preparations using twitch tension method. The isolated nerves were stimulated by electrical stimulator and response to each stimulus was recorded. The venom mainly acted prejunctionally to facilitate neuromuscular activity due to an increase in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 162 2 شماره
صفحات -
تاریخ انتشار 1977